Screening is done to determine which animals produce the highest levels of antigen-specific polyclonal antibodies. After mice are selected and processed for monoclonal antibody optimization (fusion of harvested spleen cells to produce hybridomas), the resulting hundreds to thousands of resulting monoclonal cell lines must be tested to determine which ones are producers of the desired specific antibody.
https://articlesoup.com/antibo....dy-isotyping-charact
