CloneJET PCR Cloning
FLEXIBLE. INNOVATIVE. FAST.
The Thermo Scientific CloneJET PCR Cloning Kit is a versatile, advanced positive selection system for high-efficiency cloning of PCR products. Ligation into the positive selection vector takes only five minutes, yielding more than 99% recombinant clones. All common laboratory E.coli strains can be directly transformed with the ligation product. The CloneJET PCR Cloning Kit is also available as a combo kit provided with DH10B competent cells.
WHY USE CLONEJET PCR CLONING KIT?
Fast–clone PCR products in as little as 5 minutes
Versatile–use with phosphorylated or non-phosphorylated DNA fragments and with either blunt-end or sticky-end PCR products
Efficient–generate more than 99% of positive recombinant clones with no cloning background
Economical–eliminate the need for expensive blue/white screening
Compatible–transform directly into all common E. coli strains including TOP10 and XL1- Blue
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